Article
Expression and characterization of a housefly cecropin gene in the methylotrophic yeast, Pichia pastoris.
Protein expression and purification - 1 Sept 2006
Jin Fengliang, Xu Xiaoxia, Zhang Wenqing, Gu Dexiang
Abstract excerpt
A 371 bp full-length cDNA (GenBank Accession No. DQ232774) was obtained from housefly Musca domestica by using degenerate primers and subsequent amplification by 5'- and 3'-RACE. The cecropin gene, Mdcec and Mdcec/6His, was cloned into expression pPICZalpha-A vector and was expressed in the methylotrophic yeast, Pichia pastoris. The recombinant Mdcec was purified using cationic exchange chromatography and 1.2mg...
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