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Article

Chemical rescue of active site mutants of S. pneumoniae surface endonuclease EndA and other nucleases of the HNH family by imidazole.

Chembiochem : a European journal of chemical biology - 19 Mar 2012

Midon Marika, Gimadutdinow Oleg, Meiss Gregor, Friedhoff Peter, Pingoud Alfred

Abstract excerpt

The His-Asn-His (HNH) motif characterizes the active sites of a large number of different nucleases such as homing endonucleases, restriction endonucleases, structure-specific nucleases and, in particular, nonspecific nucleases. Several biochemical studies have revealed an essential catalytic function for the first amino acid of this motif in HNH nucleases. This histidine residue was identified as the general...

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PMID: 22344704
DOI: 10.1002/cbic.201100775

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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