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Article

Mutation of an atypical oxirane oxyanion hole improves regioselectivity of the α/β-fold epoxide hydrolase Alp1U.

The Journal of biological chemistry - 11 Dec 2020

Zhang Liping, De Bidhan Chandra, Zhang Wenjun, Mándi Attila, Fang Zhuangjie, Yang Chunfang, Zhu Yiguang, Kurtán Tibor, Zhang Changsheng

Abstract excerpt

Epoxide hydrolases (EHs) have been characterized and engineered as biocatalysts that convert epoxides to valuable chiral vicinal diol precursors of drugs and bioactive compounds. Nonetheless, the regioselectivity control of the epoxide ring opening by EHs remains challenging. Alp1U is an α/β-fold EH that exhibits poor regioselectivity in the epoxide hydrolysis of fluostatin C (compound 1) and produces a pair of...

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Identifiers

PMID: 33004437
DOI: 10.1074/jbc.RA120.015563
PMCID: PMC7863881

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