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Article

Engineering novel Lec1 glycosylation mutants in CHO-DUKX cells: molecular insights and effector modulation of N-acetylglucosaminyltransferase I.

Biotechnology and bioengineering - 1 Jul 2012

Zhong Xiaotian, Cooley Cecilia, Seth Nilufer, Juo Z Sean, Presman Ella, Resendes Nicole, Kumar Ravi, Allen Martin, Mosyak Lidia, Stahl Mark, Somers Will, Kriz Ronald

Abstract excerpt

Many secreted or cell surface proteins are post-translationally modified by carbohydrate chains which are a primary source of heterogeneity. The Lec1 mutant, which is defective in Golgi N-acetylglucosaminyltransferase I (GnTI) activity, produces relatively homogeneous Man(5) GlcNAc(2) glycan modifications, and is widely used for various applications. To facilitate the investigation of GnTI, its Man5 glycan...

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PMID: 22252477
DOI: 10.1002/bit.24448

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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