Article
Image analysis of Feulgen-stained c-H-ras-transformed NIH/3T3 cells.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 2000
Mello M L, Russo J
Abstract excerpt
Feulgen-DNA content, nuclear phenotypes, and levels of chromatin condensation were evaluated by image analysis in NIH/3T3 cells transformed with the c-H-ras oncogene of T24 cells. Three nuclear phenotypes, differing from those of untransformed control cells and defined in terms of patterns of chromatin condensation, were demonstrated microspectrophotometrically for the tumor cells. Polyploidy could only be...
Topics
- Animals
- Cell Line, Transformed
- Cell Nucleus
- Cell Transformation, Neoplastic
- Chromatin
- Coloring Agents
- DNA, Neoplasm
- Genes, ras
- Image Processing, Computer-Assisted
- Mice
- Mice, Nude