Article
Peptide nucleic acid molecular beacons for the detection of PCR amplicons in droplet-based microfluidic devices.
Analytical and bioanalytical chemistry - 1 Jan 2013
Zanoli Laura Maria, Licciardello Marco, D'Agata Roberta, Lantano Claudia, Calabretta Alessandro, Corradini Roberto, Marchelli Rosangela, Spoto Giuseppe
Abstract excerpt
The use of droplet-based microfluidics and peptide nucleic acid molecular beacons for the detection of polymerase chain reaction (PCR)-amplified DNA sequences within nanoliter-sized droplets is described in this work. The nanomolar-attomolar detection capabilities of the method were preliminarily tested by targeting two different single-stranded DNA sequences from the genetically modified Roundup Ready soybean...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
