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Article

High-resolution melting genotyping of Enterococcus faecium based on multilocus sequence typing derived single nucleotide polymorphisms.

PloS one - 1 Jan 2011

Tong Steven Y C, Xie Shirley, Richardson Leisha J, Ballard Susan A, Dakh Farshid, Grabsch Elizabeth A, Grayson M Lindsay, Howden Benjamin P, Johnson Paul D R, Giffard Philip M

Abstract excerpt

We have developed a single nucleotide polymorphism (SNP) nucleated high-resolution melting (HRM) technique to genotype Enterococcus faecium. Eight SNPs were derived from the E. faecium multilocus sequence typing (MLST) database and amplified fragments containing these SNPs were interrogated by HRM. We tested the HRM genotyping scheme on 85 E. faecium bloodstream isolates and compared the results with MLST,...

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Share to Electrophoresis, Gel, Pulsed-Field
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 22195020
DOI: 10.1371/journal.pone.0029189
PMCID: PMC3241712

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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