Article
Rapid high-resolution melting genotyping scheme for Escherichia coli based on MLST derived single nucleotide polymorphisms.
Scientific reports - 16 Aug 2021
Bezdicek Matej, Nykrynova Marketa, Sedlar Karel, Kralova Stanislava, Hanslikova Jana, Komprdova Aja, Skutkova Helena, Kocmanova Iva, Mayer Jiri, Lengerova Martina
Abstract excerpt
Routinely used typing methods including MLST, rep-PCR and whole genome sequencing (WGS) are time-consuming, costly, and often low throughput. Here, we describe a novel mini-MLST scheme for Eschericha coli as an alternative method for rapid genotyping. Using the proposed mini-MLST scheme, 10,946 existing STs were converted into 1,038 Melting Types (MelTs). To validate the new mini-MLST scheme, in silico analysis...
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