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Article

Upregulation of RNase E activity by mutation of a site that uncompetitively interferes with RNA binding.

RNA biology - 1 Jan 2000

Go Hayoung, Moore Christopher J, Lee Minho, Shin Eunkyoung, Jeon Che Ok, Cha Chang-Jun, Han Seung Hyun, Kim Su-Jin, Lee Sang-Won, Lee Younghoon, Ha Nam-Chul, Kim Yong-Hak, Cohen Stanley N, Lee Kangseok

Abstract excerpt

Escherichia coli RNase E contains a site that selectively binds to RNAs containing 5'-monophosphate termini, increasing the efficiency of endonucleolytic cleavage of these RNAs. Random mutagenesis of N-Rne, the N-terminal catalytic region of RNase E, identified a hyperactive variant that remains preferentially responsive to phosphorylation at 5' termini. Biochemical analyses showed that the mutation (Q36R), which...

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Identifiers

PMID: 22186084
DOI: 10.4161/rna.8.6.18063
PMCID: PMC3360077

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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