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Article

Real-time PCR and high-resolution melt analysis for rapid detection of Mycobacterium leprae drug resistance mutations and strain types.

Journal of clinical microbiology - 1 Mar 2012

Li Wei, Matsuoka Masanori, Kai Masanori, Thapa Pratibha, Khadge Saraswoti, Hagge Deanna A, Brennan Patrick J, Vissa Varalakshmi

Abstract excerpt

Drug resistance surveillance and strain typing of Mycobacterium leprae are necessary to investigate ongoing transmission of leprosy in regions of endemicity. To enable wider implementation of these molecular analyses, novel real-time PCR-high-resolution melt (RT-PCR-HRM) assays without allele-specific primers or probes and post-PCR sample handling were developed. For the detection of mutations within drug...

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Identifiers

PMID: 22170923
DOI: 10.1128/JCM.05183-11
PMCID: PMC3295127

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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