Article
High-throughput detection of highly benzimidazole-resistant allele E198A with mismatch primers in allele-specific real-time polymerase chain reaction.
Pest management science - 1 Apr 2009
Chen Changjun, Zhao Wei, Lu Yuejian, Wang Jianxin, Chen Yu, Li Hongxia, Zhou Mingguo
Abstract excerpt
BACKGROUND: A point mutation often confers resistance of organisms against medical drugs and agricultural pesticides. Allele-specific nucleotide polymerase chain reaction (ASPCR) and allele-specific quantitative real-time PCR using SYBR Green (ASQPCR) are widely and effectively applied to detect...
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