Heterologous gene expression on the linear DNA killer plasmid from Kluyveromyces lactis.

doi:10.1007/BF00326291

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Article

Heterologous gene expression on the linear DNA killer plasmid from Kluyveromyces lactis.

Current genetics - 1 Feb 1991

Kämper J, Esser K, Gunge N, Meinhardt F

Abstract excerpt

Linear hybrid plasmids based on the killer plasmid pGKL1 from Kluyveromyces lactis were obtained by in vivo recombination in Saccharomyces cerevisiae. Like pGKL1, the hybrids are located in the cytoplasm, have terminal inverted repeats (TIR) and possess covalently linked proteins at their 5' ends...

Topics

Base Sequence
Blotting, Northern
Cloning, Molecular
DNA, Fungal
Gene Expression
Kluyveromyces
Molecular Sequence Data
Phenotype
Plasmids
Promoter Regions, Genetic
Recombination, Genetic
Restriction Mapping
Transcription, Genetic
Transformation, Genetic

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