Article
Expression of a foreign KmR gene in linear killer DNA plasmids in yeast.
Gene - 2 Jul 1990
Tanguy-Rougeau C, Chen X J, Wésolowski-Louvel M, Fukuhara H
Abstract excerpt
The killer plasmids of the yeast Kluyveromyces lactis, pGKL1 and 2 (k1 and k2 for short), are linear double-stranded DNAs. The expression of genes of these plasmids is thought to depend on their own transcription system. Cloning the plasmid genes in conventional circular vectors is therefore not suitable for transcriptional studies, because such vectors use the host nuclear transcription system. In vitro...
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