Article
Expression of a foreign KmR gene in linear killer DNA plasmids in yeast.
Gene - 2 Jul 1990
Tanguy-Rougeau C, Chen X J, Wésolowski-Louvel M, Fukuhara H
Abstract excerpt
The killer plasmids of the yeast Kluyveromyces lactis, pGKL1 and 2 (k1 and k2 for short), are linear double-stranded DNAs. The expression of genes of these plasmids is thought to depend on their own transcription system. Cloning the plasmid genes in conventional circular vectors is therefore not...
Topics
- DNA, Fungal
- Genes, Fungal
- Kluyveromyces
- Phenotype
- Plasmids
- Recombination, Genetic
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomycetales
- Transformation, Genetic
