Article
Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae.
Bioscience, biotechnology, and biochemistry - 1 May 1996
Kishida M, Tokunaga M, Katayose Y, Yajima H, Kawamura-Watabe A, Hishinuma F
Abstract excerpt
The linear double stranded DNA plasmid pGKL1 encodes the yeast killer toxin complex (Gunge et al., 1981) of which the killing mechanism is not understood. We isolated and characterized eight mutants in Saccharomyces cerevisiae that were insensitive to both the intracellularly expressed 28-kDa kil...
Topics
- Cloning, Molecular
- DNA
- DNA, Fungal
- Fungal Proteins
- Gene Library
- Genetic Complementation Test
- Killer Factors, Yeast
- Molecular Weight
- Mutation
- Mycotoxins
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins