Article
Secretion of functional papain precursor from insect cells. Requirement for N-glycosylation of the pro-region.
The Journal of biological chemistry - 25 Sept 1990
Vernet T, Tessier D C, Richardson C, Laliberté F, Khouri H E, Bell A W, Storer A C, Thomas D Y
Abstract excerpt
The synthetic gene coding for the precursor of the cysteine protease papain (EC 3.4.22.2) has been expressed using the baculovirus/insect cell system. The prepropapain gene was cloned into the transfer vector IpDC125 behind the polyhedrin promoter. The recombinant construct was then incorporated...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Enzyme Precursors
- Genes, Synthetic
- Glycosylation
- Insect Viruses
- Molecular Sequence Data
- Moths
- Mutation
- Oligonucleotide Probes