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Article

Secretion of functional papain precursor from insect cells. Requirement for N-glycosylation of the pro-region.

The Journal of biological chemistry - 25 Sept 1990

Vernet T, Tessier D C, Richardson C, Laliberté F, Khouri H E, Bell A W, Storer A C, Thomas D Y

Abstract excerpt

The synthetic gene coding for the precursor of the cysteine protease papain (EC 3.4.22.2) has been expressed using the baculovirus/insect cell system. The prepropapain gene was cloned into the transfer vector IpDC125 behind the polyhedrin promoter. The recombinant construct was then incorporated by homologous recombination into the Autographa californiaca nuclear polyhedrosis virus genome. The host Spodoptera...

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PMID: 2204628

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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