Article
13-cis retinoic acid augments the production of macrophages in mouse bone marrow cultures stimulated with interleukin 3.
Experimental hematology - 1 Oct 1990
Bender J G, Stewart C C, Van Epps D E, Walker D E
Abstract excerpt
Mouse bone marrow cells in liquid culture with interleukin 3 generate nonadherent granulocytes, mast cells, and macrophages. The addition of 13-cis retinoic acid (13cRA) (10(-8)-10(-6) M) enhanced proliferation of the nonadherent cells, and concentrations greater than 5 x 10(-7) M stimulated a sixfold increase in adherent macrophages. Four-color flow cytometry was used to identify the lineages present using the...
Topics
- Animals
- Bone Marrow
- Cell Division
- Cells, Cultured
- Colony-Stimulating Factors
- Flow Cytometry
- Granulocyte-Macrophage Colony-Stimulating Factor
- Growth Substances
- Hematopoiesis
- Interleukin-3
- Isotretinoin