Cleavages in the 5' region of the ompA and bla mRNA control stability: studies with an E. coli mutant altering mRNA stability and a novel endoribonuclease.

doi:10.1002/j.1460-2075.1990.tb07460.x

Article

Cleavages in the 5' region of the ompA and bla mRNA control stability: studies with an E. coli mutant altering mRNA stability and a novel endoribonuclease.

The EMBO journal - 1 Sept 1990

Lundberg U, von Gabain A, Melefors O

Abstract excerpt

We describe here the partial purification of a novel Escherichia coli endoribonuclease, RNase K. This protein catalyses site-specific cleavages in the 5' region of in vitro transcribed ompA and bla transcripts. Some of the resulting cleavage products are also found in cellular ompA mRNA, defining...

Topics

Bacterial Outer Membrane Proteins
Base Sequence
Endoribonucleases
Escherichia coli
Models, Molecular
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Oligonucleotide Probes
Plasmids
RNA, Messenger
Transcription, Genetic
beta-Lactamases

Cleavages in the 5' region of the ompA and bla mRNA control stability: studies with an E. coli mutant altering mRNA stability and a novel endoribonuclease.

Abstract excerpt

Topics

Discuss the evidence