Article
[Polymerase chain reaction (PCR)--a novel tool for the molecular diagnosis of neoplasms].
Gan to kagaku ryoho. Cancer & chemotherapy - 1 Aug 1990
Yokota S, Abe T
Abstract excerpt
Polymerase chain reaction (PCR) is a novel tool for the in vitro amplification of DNA segments up to several kb. Repeated cycles of DNA synthesis by heat-stable Taq DNA polymerase enables to obtain more than 10(5) copies of the target sequence. Recently its enormous attitude of amplification has been applied for the detection of tumor-specific gene alterations. Examples include the detection of point mutation of...
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