Article
[Pathology--on a molecular level (determination of nucleic acids from fixed embedded samples)].
Orvosi hetilap - 12 Jul 1992
Kopper L, Kovalszky I
Abstract excerpt
Molecular biological techniques may open new avenues to pathological archives. Fixed and paraffin embedded blocks are a suitable source of nucleic acids, especially of DNA, for retrospective analysis. The quality of DNA depends mainly on the fixation procedure. High molecular weight DNA allows Southern hybridization, but fragmented DNA also became a target with the appearance of polymerase chain reaction (PCR)....
Topics
- Blotting, Northern
- Blotting, Southern
- DNA, Neoplasm
- Electrophoresis
- Gene Expression
- Hodgkin Disease
- Humans
- Lymphoma, Non-Hodgkin
- Molecular Biology
- Nucleic Acid Hybridization
- Phenotype