Article
Experimental evolution of a facultative thermophile from a mesophilic ancestor.
Applied and environmental microbiology - 1 Jan 2012
Blaby Ian K, Lyons Benjamin J, Wroclawska-Hughes Ewa, Phillips Grier C F, Pyle Tyler P, Chamberlin Stephen G, Benner Steven A, Lyons Thomas J, Crécy-Lagard Valérie de, Crécy Eudes de
Abstract excerpt
Experimental evolution via continuous culture is a powerful approach to the alteration of complex phenotypes, such as optimal/maximal growth temperatures. The benefit of this approach is that phenotypic selection is tied to growth rate, allowing the production of optimized strains. Herein, we demonstrate the use of a recently described long-term culture apparatus called the Evolugator for the generation of a...
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