Mutagenicity test for unstable compounds, such as 5,6-dihydroxyindole, using an Escherichia coli HB101/pBR322 transfection system.

doi:10.1093/mutage/5.3.251

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Article

Mutagenicity test for unstable compounds, such as 5,6-dihydroxyindole, using an Escherichia coli HB101/pBR322 transfection system.

Mutagenesis - 1 May 1990

Miranda M, Amicarelli F, Bonfigli A, Poma A, Zarivi O, Arcadi A

Abstract excerpt

A mutagenicity test for unstable chemical compounds has been devised. The test makes use of (i) in vitro treatment of plasmid pBR322 with the putative mutagen (ii) subsequent transfection of Escherichia coli HB101; (iii) selection either on tetracycline- or ampicillin-containing Eugon agar (iv) c...

Topics

Ampicillin Resistance
Escherichia coli
Indoles
Mutagenicity Tests
Mutation
Plasmids
Restriction Mapping
Tetracycline Resistance
Transfection

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