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Article

Expression, purification, and crystallization of natural and selenomethionyl recombinant ribonuclease H from Escherichia coli.

The Journal of biological chemistry - 15 Aug 1990

Yang W, Hendrickson W A, Kalman E T, Crouch R J

Abstract excerpt

Ribonuclease H (RNase H) from Escherichia coli is an endonuclease that specifically degrades the RNAs of RNA:DNA hybrids. The enzyme is a single polypeptide chain of 155 amino acid residues, of which 4 are methionines. To solve the crystallographic three-dimensional structure of E. coli RNase H by the multi-wavelength anomalous diffraction technique, we have constructed methionine auxotrophic strains of E. coli...

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PMID: 2199440

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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