Article
The interaction of Pcf11 and Clp1 is needed for mRNA 3'-end formation and is modulated by amino acids in the ATP-binding site.
Nucleic acids research - 1 Feb 2012
Ghazy Mohamed A, Gordon James M B, Lee Susan D, Singh Badri Nath, Bohm Andrew, Hampsey Michael, Moore Claire
Abstract excerpt
Polyadenylation of eukaryotic mRNAs contributes to stability, transport and translation, and is catalyzed by a large complex of conserved proteins. The Pcf11 subunit of the yeast CF IA factor functions as a scaffold for the processing machinery during the termination and polyadenylation of transcripts. Its partner, Clp1, is needed for mRNA processing, but its precise molecular role has remained enigmatic. We show...
Topics
- Adenosine Triphosphate
- Amino Acid Substitution
- Binding Sites
- Mutation
- Phenotype
- Polyadenylation
- Protein Structure, Tertiary
- Protein Subunits
- RNA 3' End Processing
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription, Genetic