Article
Induction of toxin-specific neutralizing immunity by molecularly uniform rice-based oral cholera toxin B subunit vaccine without plant-associated sugar modification.
Plant biotechnology journal - 1 Sept 2013
Yuki Yoshikazu, Mejima Mio, Kurokawa Shiho, Hiroiwa Tomoko, Takahashi Yuko, Tokuhara Daisuke, Nochi Tomonori, Katakai Yuko, Kuroda Masaharu, Takeyama Natsumi, Kashima Koji, Abe Michiyo, Chen Yingju, Nakanishi Ushio, Masumura Takehiro, Takeuchi Yoji, Kozuka-Hata Hiroko, Shibata Hiroaki, Oyama Masaaki, Tanaka Kunisuke, Kiyono Hiroshi
Abstract excerpt
Plants have been used as expression systems for a number of vaccines. However, the expression of vaccines in plants sometimes results in unexpected modification of the vaccines by N-terminal blocking and sugar-chain attachment. Although MucoRice-CTB was thought to be the first cold-chain-free and unpurified oral vaccine, the molecular heterogeneity of MucoRice-CTB, together with plant-based sugar modifications of...
Topics
- Animals
- Antibodies, Neutralizing
- Cholera Toxin
- Cholera Vaccines
- Electrophoresis, Polyacrylamide Gel
- Female
- Immunization
- Macaca