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Article

Translational activation of GCN4 mRNA in a cell-free system is triggered by uncharged tRNAs.

Molecular and cellular biology - 1 Aug 1990

Krupitza G, Thireos G

Abstract excerpt

Translation of GCN4 mRNA is activated when yeast cells are grown under conditions of amino acid limitation. In this study, we established the conditions through which translation of the GCN4 mRNA could be activated in a homologous in vitro system. This activation paralleled the in vivo situation: it required the small open reading frames located in the 5' untranslated region of the GCN4 mRNA, and it was coupled...

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Identifiers

PMID: 2196452
DOI: 10.1128/mcb.10.8.4375-4378.1990
PMCID: PMC360992

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Translational activation of GCN4 mRNA in a cell-free system is triggered by uncharged tRNAs.PMID 2196452Read ↗

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cis-acting sequences involved in the translational control of GCN4 expression.Semantic proximity Read ↗

GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, is required for phosphorylation of eukaryotic translation initiation factor 2 by protein kinase GCN2.Semantic proximity Read ↗

Identification of the mRNA targets of tRNA-specific regulation using genome-wide simulation of translation.Semantic proximity Read ↗

Separate domains in GCN1 for binding protein kinase GCN2 and ribosomes are required for GCN2 activation in amino acid-starved cells.Semantic proximity Read ↗

Identification of positive-acting domains in GCN2 protein kinase required for translational activation of GCN4 expression.Semantic proximity Read ↗

Yeast phenotypic assays on translational control.Semantic proximity Read ↗

Mutations that bypass tRNA binding activate the intrinsically defective kinase domain in GCN2.Semantic proximity Read ↗

Defects in tRNA processing and nuclear export induce GCN4 translation independently of phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2.Semantic proximity Read ↗

Translational activation of uncapped mRNAs by the central part of human eIF4G is 5′ end-dependentSemantic proximity Read ↗

Utilizing the GCN4 leader region to investigate the role of the sequence determinants in nonsense-mediated mRNA decay.Semantic proximity Read ↗

The yeast eIF4E-associated protein Eap1p attenuates GCN4 translation upon TOR-inactivation.Semantic proximity Read ↗

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