Article
Solution structure of proinsulin: connecting domain flexibility and prohormone processing.
The Journal of biological chemistry - 12 Mar 2010
Yang Yanwu, Hua Qing-Xin, Liu Jin, Shimizu Eri H, Choquette Meredith H, Mackin Robert B, Weiss Michael A
Abstract excerpt
The folding of proinsulin, the single-chain precursor of insulin, ensures native disulfide pairing in pancreatic beta-cells. Mutations that impair folding cause neonatal diabetes mellitus. Although the classical structure of insulin is well established, proinsulin is refractory to crystallization. Here, we employ heteronuclear NMR spectroscopy to characterize a monomeric analogue. Proinsulin contains a...
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