Article
Efficient site directed in vitro mutagenesis using ampicillin selection.
Nucleic acids research - 25 Jun 1990
Lewis M K, Thompson D V
Abstract excerpt
A novel plasmid vector pSELECT-1 is described which can be used for highly efficient site-directed in vitro mutagenesis. The mutagenesis method is based on the use of single-stranded DNA and two primers, one mutagenic primer and a second correction primer which corrects a defect in the ampicillin resistance gene on the vector and reverts the vector to ampicillin resistance. Using T4 DNA polymerase and T4 DNA...
Topics
- Ampicillin Resistance
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Phosphorylation
- Plasmids