Article
Cloning of spiramycin biosynthetic genes and their use in constructing Streptomyces ambofaciens mutants defective in spiramycin biosynthesis.
Journal of bacteriology - 1 Jul 1990
Richardson M A, Kuhstoss S, Huber M L, Ford L, Godfrey O, Turner J R, Rao R N
Abstract excerpt
Several cosmid clones from Streptomyces ambofaciens containing the spiramycin resistance gene srmB were introduced into S. fradiae PM73, a mutant defective in tylosin synthesis, resulting in tylosin synthesis. The DNA responsible for this complementation was localized to a 10.5-kilobase EcoRI fragment. A 32-kilobase DNA segment which included the srmB spiramycin resistance gene and DNA which complemented the...
Topics
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA, Bacterial
- DNA, Recombinant
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Bacterial
- Mutation
- Plasmids
- Restriction Mapping