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Article

A more efficient method to generate null mutants using Hprt-Cre with floxed alleles.

Journal of pediatric surgery - 1 Sept 2011

Nichol Peter F, Botham Robert, Saijoh Yukio, Reeder Amy L, Zaremba Krzyztoff M

Abstract excerpt

PURPOSE: The generation of nonviable homozygous null mouse embryos from heterozygote null/+ breedings can be highly resource consuming, with only 25% of the embryos in the litter being null mutants. We hypothesized that (1) we could double the number of homozygous null mouse embryos in a litter without reducing litter size using Hypoxanthine-guanine phosphoribosyltransferase-Cre (Hprt)-Cre (which is active in the...

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Identifiers

PMID: 21929979
DOI: 10.1016/j.jpedsurg.2011.01.023
PMCID: PMC3177085

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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