Article
Human immunodeficiency virus type 1 drug resistance testing: Evaluation of a new ultra-deep sequencing-based protocol and comparison with the TRUGENE HIV-1 Genotyping Kit.
Journal of virological methods - 1 Dec 2011
Stelzl Evelyn, Pröll Johannes, Bizon Beata, Niklas Norbert, Danzer Martin, Hackl Christa, Stabentheiner Stephanie, Gabriel Christian, Kessler Harald H
Abstract excerpt
Genotypic HIV-1 drug resistance testing with standard Sanger sequencing is limited to the detection of mutations with >20% prevalence. A new protocol for variant detection of protease and reverse transcriptase genes of HIV-1 genotype B samples with ultra-deep sequencing on the GS-FLX sequencer (Roche 454 Life Sciences, Branford, CT) was evaluated. The new technology was compared with the standard Sanger...
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