Article
Escherichia coli metR mutants that produce a MetR activator protein with an altered homocysteine response.
Journal of bacteriology - 1 Jun 1990
Byerly K A, Urbanowski M L, Stauffer G V
Abstract excerpt
Using an Escherichia coli lac deletion strain lysogenized with a lambda phage carrying a metH-lacZ gene fusion, we isolated trans-acting mutations that result in simultaneous 4- to 6-fold-elevated metH-lacZ expression, 5- to 22-fold-lowered metE-lacZ expression, and 9- to 20-fold-elevated metR-lacZ expression. The altered regulation of these genes occurs in the presence of high intracellular levels of...
Topics
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Homocysteine
- Lac Operon
- Methionine
- Mutation
- Trans-Activators
- Transduction, Genetic