Article
Development of a PCR-based method for specific identification of genotypic markers of shiga toxin-producing Escherichia coli strains.
Journal of veterinary medicine. B, Infectious diseases and veterinary public health - 1 Dec 2001
Osek J, Dacko J
Abstract excerpt
A simple, rapid and specific PCR-based method for identification of shiga toxin-producing Escherichia coli (STEC) was developed. The procedure involves amplification of the E. coli-specific universal stress protein A (uspA) gene (uspa-PCR), with the primer pair described by other authors, which allows differentiation of E. coli (STEC and non-STEC) from other gram-negative bacteria followed by identification of...
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