Article
Programmed ribosomal frameshifting generates the Escherichia coli DNA polymerase III gamma subunit from within the tau subunit reading frame.
Nucleic acids research - 11 Apr 1990
Blinkowa A L, Walker J R
Abstract excerpt
The Escherichia coli dnaX gene encodes both the tau and gamma subunits of DNA polymerase III holoenzyme in one reading frame. The 71.1 kDa tau and the shorter gamma share N-terminal sequences. Mutagenesis of a potential ribosomal frameshift signal located at codons 428-430 without changing the amino acid sequence of the tau product, eliminated detectable synthesis of the gamma subunit, suggesting that the reading...
Topics
- Amino Acid Sequence
- Base Sequence
- Codon
- DNA Polymerase III
- DNA, Recombinant
- DNA-Directed DNA Polymerase
- Escherichia coli
- Genes, Bacterial
- Macromolecular Substances
- Molecular Sequence Data
- Mutation