Second-site revertants of the P1 copN22 copy mutant.

doi:10.1128/jb.172.5.2762-2764.1990

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Article

Second-site revertants of the P1 copN22 copy mutant.

Journal of bacteriology - 1 May 1990

Froehlich B J, Scott J R

Abstract excerpt

Miniplasmids with the P1 copN22 mutation have a copy number about seven times that of the wild type. Selection for reduced copy number from this plasmid led to the isolation of second-site pseudorevertants, called poc mutants. DNA sequence analysis showed that all six independent poc mutants have...

Topics

Base Sequence
Codon
DNA, Bacterial
Escherichia coli
Molecular Sequence Data
Mutation
Plasmids
Restriction Mapping

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