Article
Substrate tRNA recognition mechanism of a multisite-specific tRNA methyltransferase, Aquifex aeolicus Trm1, based on the X-ray crystal structure.
The Journal of biological chemistry - 7 Oct 2011
Awai Takako, Ochi Anna, Ihsanawati, Sengoku Toru, Hirata Akira, Bessho Yoshitaka, Yokoyama Shigeyuki, Hori Hiroyuki
Abstract excerpt
Archaeal and eukaryotic tRNA (N(2),N(2)-guanine)-dimethyltransferase (Trm1) produces N(2),N(2)-dimethylguanine at position 26 in tRNA. In contrast, Trm1 from Aquifex aeolicus, a hyper-thermophilic eubacterium, modifies G27 as well as G26. Here, a gel mobility shift assay revealed that the T-arm in tRNA is the binding site of A. aeolicus Trm1. To address the multisite specificity, we performed an x-ray crystal...
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