Point mutations in a pBR322-based expression plasmid resulting in increased synthesis of bovine growth hormone in Escherichia coli.

doi:10.1016/0378-1119(90)90272-s

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Article

Point mutations in a pBR322-based expression plasmid resulting in increased synthesis of bovine growth hormone in Escherichia coli.

Gene - 14 Feb 1990

Watson N, Olson E R

Abstract excerpt

The bGH cDNA coding for bovine growth hormone (bGH) is expressed poorly in Escherichia coli using a pBR322-based expression plasmid. Random mutagenesis of the plasmid gave rise to two types of plasmid mutants which increased the expression of bGH. One class had single base changes in the first fo...

Topics

Amino Acid Sequence
Animals
Base Sequence
Cattle
Cloning, Molecular
DNA
Escherichia coli
Gene Expression
Growth Hormone
Molecular Sequence Data
Mutation
Plasmids
Restriction Mapping

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