Article
Domain complementation studies reveal residues critical for the activity of the mannitol permease from Escherichia coli.
Biochimica et biophysica acta - 1 Feb 2009
Vos Erwin P P, ter Horst Ramon, Poolman Bert, Broos Jaap
Abstract excerpt
This paper presents domain complementation studies in the mannitol transporter, EIImtl, from Escherichia coli. EIImtl is responsible for the transport and concomitant phosphorylation of mannitol over the cytoplasmic membrane. By using tryptophan-less EIImtl as a basis, each of the four phenylalanines located in the cytoplasmic loop between putative transmembrane helices II and III in the membrane-embedded C...
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