Article
Photocrosslinking of benzophenone-labeled single cysteine troponin I mutants to other thin filament proteins.
Journal of molecular biology - 25 Feb 2000
Luo Y, Wu J L, Li B, Langsetmo K, Gergely J, Tao T
Abstract excerpt
The interaction sites of rabbit skeletal troponin I (TnI) with troponin C (TnC), troponin T (TnT), tropomyosin (Tm) and actin were mapped systematically using nine single cysteine residue TnI mutants with mutation sites at positions 6, 48, 64, 89, 104, 121, 133, 155 or 179 (TnI6, TnI48 etc.). Each mutant was labeled with the heterobifunctional photocrosslinker 4-maleimidobenzophenone (BP-Mal), and incorporated...
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