Cloning and nucleotide sequence of bisC, the structural gene for biotin sulfoxide reductase in Escherichia coli.

Abstract excerpt

Clones of the Escherichia coli bisC locus have been isolated by complementing a bisC mutant for growth with d-biotin d-sulfoxide as a biotin source. The complementation properties of deletions and Tn5 insertions located the bisC gene to a 3.7-kilobase-pair (kbp) segment, 3.3 kbp of which has been sequenced. A single open reading frame of 2,178 bp, capable of encoding a polypeptide of molecular weight 80,905, was...

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• Escherichia coli
• Genes, Bacterial
• Genetic Complementation Test
• Molecular Sequence Data
• Mutation
• Oxidoreductases
• Restriction Mapping