Molecular analysis of growth inhibition caused by overexpression of the biotin operon in Escherichia coli.

doi:10.1271/bbb.59.184

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Article

Molecular analysis of growth inhibition caused by overexpression of the biotin operon in Escherichia coli.

Bioscience, biotechnology, and biochemistry - 1 Feb 1995

Ifuku O, Koga N, Haze S, Kishimoto J, Arai T, Wachi Y

Abstract excerpt

Constitutive overexpression of the biotin operon (type 9 mutation) in a multicopy plasmid resulted in growth inhibition in Escherichia coli. Deletion analysis of the biotin operon indicated that overexpression of the bioB gene alone, the product of which is believed to catalyze the conversion of...

Topics

Amino Acid Sequence
Base Sequence
Biotin
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Gene Expression Regulation, Bacterial
Molecular Sequence Data
Mutation
Operon
Plasmids

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