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Cloning and nucleotide sequencing of the secA gene from coryneform bacteria.

Genetic analysis : biomolecular engineering - 1 Mar 1999

Kobayashi M, Fugono N, Asai Y, Yukawa H

Abstract excerpt

Taking advantage of highly conserved domains present in the secA gene from Escherichia coli and Bacillus subtilis, we designed degenerate oligonucleotides (oligos) corresponding to these regions. These oligos were used as primers in PCR in order to amplify DNA sequences from Brevibacterium flavum MJ233 chromosomal DNA. The PCR product was used as a probe to recover genomic fragments from a lambda library of Br....

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PMID: 10084122
DOI: 10.1016/s1050-3862(98)00010-2

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