Article
Cloning and nucleotide sequencing of the secA gene from coryneform bacteria.
Genetic analysis : biomolecular engineering - 1 Mar 1999
Kobayashi M, Fugono N, Asai Y, Yukawa H
Abstract excerpt
Taking advantage of highly conserved domains present in the secA gene from Escherichia coli and Bacillus subtilis, we designed degenerate oligonucleotides (oligos) corresponding to these regions. These oligos were used as primers in PCR in order to amplify DNA sequences from Brevibacterium flavum MJ233 chromosomal DNA. The PCR product was used as a probe to recover genomic fragments from a lambda library of Br....
Topics
- Adenosine Triphosphatases
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Corynebacterium
- DNA Primers
- Escherichia coli Proteins
- Genes, Bacterial
- Genetic Complementation Test
- Genotype
- Membrane Transport Proteins
- Molecular Sequence Data
- SEC Translocation Channels
- SecA Proteins
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Temperature