Identification and characterization of a new disulfide isomerase-like protein (DsbD) in Escherichia coli.

Abstract excerpt

Previous studies have established that DsbA and DsbC, periplasmic proteins of Escherichia coli, are two key players involved in disulfide bond formation. A search for extragenic mutations able to compensate for the lack of dsbA function in vivo led us to the identification of a new gene, designated dsbD. Lack of DsbD protein leads to some, but not all, of the phenotypic defects observed with other dsb mutations,...

Topics

• Amino Acid Sequence
• Base Sequence
• Chromosome Mapping
• Cloning, Molecular
• DNA Primers
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Genes, Bacterial
• Isomerases
• Molecular Sequence Data
• Mutation