Article
Transposon insertion mutagenesis of a genetic region encoding serum resistance in an 80 kb plasmid of Salmonella dublin.
Journal of general microbiology - 1 Sept 1990
Terakado N, Ushijima T, Samejima T, Ito H, Hamaoka T, Murayama S, Kawahara K, Danbara H
Abstract excerpt
Using transposon insertion mutagenesis with Tn1 or Tn5, we obtained Salmonella dublin mutant strains that showed either diminished serum resistance (five mutants) or diminished mouse lethality (two mutants). Detailed restriction cleavage analysis to determine the single sites of transposon insert...
Topics
- Animals
- Blood Bactericidal Activity
- Carbohydrates
- DNA Transposable Elements
- DNA, Bacterial
- Lipopolysaccharides
- Mice
- Mutagenesis
- Phenotype
- Plasmids
- Rabbits
- Restriction Mapping
- Salmonella
- Virulence