Article
Concanavalin A produces a matrix-degradative phenotype in human fibroblasts. Induction and endogenous activation of collagenase, 72-kDa gelatinase, and Pump-1 is accompanied by the suppression of the tissue inhibitor of matrix metalloproteinases.
The Journal of biological chemistry - 5 Dec 1990
Overall C M, Sodek J
Abstract excerpt
The lectin concanavalin A (ConA) causes fibroblasts to acquire an arborized morphology and to express elevated levels of collagenase. The temporal and mechanistic aspects of ConA regulation of matrix metalloproteinases (MMPs) and the tissue inhibitor of matrix metalloproteinases (TIMP) were chara...
Topics
- Blotting, Northern
- Concanavalin A
- Enzyme Activation
- Enzyme Induction
- Fibroblasts
- Gelatinases
- Gene Expression
- Humans
- Kinetics
- Microbial Collagenase
- Molecular Weight
- Neoplasm Proteins
- Nucleic Acid Hybridization
- Pepsin A
- Phenotype
- RNA, Messenger
- Tissue Inhibitor of Metalloproteinase-2