Article
Translocation channel gating kinetics balances protein translocation efficiency with signal sequence recognition fidelity.
Molecular biology of the cell - 1 Sept 2011
Trueman Steven F, Mandon Elisabet C, Gilmore Reid
Abstract excerpt
The transition between the closed and open conformations of the Sec61 complex permits nascent protein insertion into the translocation channel. A critical event in this structural transition is the opening of the lateral translocon gate that is formed by four transmembrane (TM) spans (TM2, TM3, TM7, and TM8 in Sec61p) to expose the signal sequence-binding site. To gain mechanistic insight into lateral gate...
Topics
- Amino Acid Motifs
- Cathepsin A
- Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
- Kinetics
- Membrane Transport Proteins
- Multiprotein Complexes
- Mutagenesis, Site-Directed
- Mutation, Missense
- Phenotype
- Protein Binding