A 62,000 molecular weight spliceosome protein crosslinks to the intron polypyrimidine tract.

doi:10.1093/nar/18.20.5995

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Article

A 62,000 molecular weight spliceosome protein crosslinks to the intron polypyrimidine tract.

Nucleic acids research - 25 Oct 1990

Wang J, Pederson T

Abstract excerpt

Incubation in HeLa nuclear extract of a 32P-labeled 61 nucleotide-long RNA corresponding to the lariat branch site/polypyrimidine tract/3' splice site of the first intron of human beta-globin pre-mRNA led to the crosslinking of a single protein of approximately 62,000 mol. wt. (p62). p62 correspo...

Topics

Base Sequence
Cell Nucleus
Cross-Linking Reagents
Endoribonucleases
Globins
HeLa Cells
Humans
Introns
Molecular Sequence Data
Molecular Weight
Mutation
Nuclear Proteins
Plasmids
Pyrimidines
RNA Precursors
RNA Splicing
Ribonuclease H
Substrate Specificity

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