Article
T4 RNA ligase 2 truncated active site mutants: improved tools for RNA analysis.
BMC biotechnology - 1 Jul 2011
Viollet Sebastien, Fuchs Ryan T, Munafo Daniela B, Zhuang Fanglei, Robb Gregory B
Abstract excerpt
BACKGROUND: T4 RNA ligases 1 and 2 are useful tools for RNA analysis. Their use upstream of RNA analyses such as high-throughput RNA sequencing and microarrays has recently increased their importance. The truncated form of T4 RNA ligase 2, comprising amino acids 1-249 (T4 Rnl2tr), is an attractive tool for attachment of adapters or labels to RNA 3'-ends. Compared to T4 RNA ligase 1, T4 Rnl2tr has a decreased...
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