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Article

New IS10 transposition vectors based on a gram-positive replication origin.

Gene - 1 Jul 1992

Mahillon J, Kleckner N

Abstract excerpt

We describe below a set of plasmid-based vehicles which can be used for delivery of IS10-derived transposons into Gram- bacteria. These vehicles replicate via a Gram+ plasmid origin that is inactive in Escherichia coli; they are easily maintained in Bacillus subtilis. Transposons are introduced by electroporation or transformation with the plasmid, and as in previous delivery systems, transpositions are selected...

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Identifiers

PMID: 1321071
DOI: 10.1016/0378-1119(92)90630-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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