New IS10 transposition vectors based on a gram-positive replication origin.

doi:10.1016/0378-1119(92)90630-8

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Article

New IS10 transposition vectors based on a gram-positive replication origin.

Gene - 1 Jul 1992

Mahillon J, Kleckner N

Abstract excerpt

We describe below a set of plasmid-based vehicles which can be used for delivery of IS10-derived transposons into Gram- bacteria. These vehicles replicate via a Gram+ plasmid origin that is inactive in Escherichia coli; they are easily maintained in Bacillus subtilis. Transposons are introduced b...

Topics

Bacillus subtilis
Base Sequence
DNA Replication
DNA Transposable Elements
Electric Stimulation
Escherichia coli
Genetic Markers
Genetic Vectors
Molecular Sequence Data
Mutation
Nucleotidyltransferases
Plasmids
Restriction Mapping
Transformation, Bacterial
Transposases

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