Article
Characterization of a mutant of Haemophilus influenzae type b lacking the P2 major outer membrane protein.
Infection and immunity - 1 Oct 1990
Cope L D, Pelzel S E, Latimer J L, Hansen E J
Abstract excerpt
An isogenic mutant of Haemophilus influenzae type b (Hib) lacking the ability to express the P2 major outer membrane (porin) protein was constructed and characterized in various model systems. Linker insertion mutagenesis of a cloned Hib DNA insert containing the P2 structural gene was used in co...
Topics
- Animals
- Bacterial Outer Membrane Proteins
- Blood Bactericidal Activity
- Blotting, Southern
- Blotting, Western
- Cloning, Molecular
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Genes
- Genes, Bacterial
- Haemophilus Infections
- Haemophilus influenzae
- Mutation
- Plasmids
- Porins
- Rats
- Suppression, Genetic
- Transformation, Bacterial