Article
Isolation of a gene for a regulatory 15-kDa subunit of mitochondrial F1F0-ATPase and construction of mutant yeast lacking the protein.
European journal of biochemistry - 28 Aug 1990
Yoshida Y, Sato T, Hashimoto T, Ichikawa N, Nakai S, Yoshikawa H, Imamoto F, Tagawa K
Abstract excerpt
A gene coding for yeast 15-kDa protein, a regulatory factor of mitochondrial F1F0-ATPase, was isolated. The cloned gene was disrupted in vitro and mutant strains that did not contain the 15-kDa protein were constructed by transformation of yeast cells with the disrupted gene. The ATP-synthesizing...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA
- DNA Transposable Elements
- Genes
- Mitochondria
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Proton-Translocating ATPases
- Saccharomyces cerevisiae