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Article

Isolation of a gene for a regulatory 15-kDa subunit of mitochondrial F1F0-ATPase and construction of mutant yeast lacking the protein.

European journal of biochemistry - 28 Aug 1990

Yoshida Y, Sato T, Hashimoto T, Ichikawa N, Nakai S, Yoshikawa H, Imamoto F, Tagawa K

Abstract excerpt

A gene coding for yeast 15-kDa protein, a regulatory factor of mitochondrial F1F0-ATPase, was isolated. The cloned gene was disrupted in vitro and mutant strains that did not contain the 15-kDa protein were constructed by transformation of yeast cells with the disrupted gene. The ATP-synthesizing activity of the mutant mitochondria was the same as that of wild-type cells, suggesting that the 15-kDa protein is not...

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PMID: 2169416
DOI: 10.1111/j.1432-1033.1990.tb19193.x

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